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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NCS1 Polyclonal antibody

NCS1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10506

Print datasheet

Synonyms

DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1

验证数据展示

  • FC experiment of SH-SY5Y using CL488-10506

    FC experiment of SH-SY5Y using CL488-10506

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NCS1 (CL488-10506) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10506 targets NCS1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NCS1 fusion protein Ag0784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name frequenin homolog (Drosophila)
Synonyms DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC004856
Gene Symbol NCS1
Gene ID (NCBI) 23413
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62166
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NCS1 antibody CL488-10506Download protocol
Standard Protocols
Click here to view our Standard Protocols