验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68954-1-Ig targets NDEL1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | NDEL1 fusion protein Ag11197 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nudE nuclear distribution gene E homolog (A. nidulans)-like 1 |
| Synonyms | |
| Calculated Molecular Weight | 345 aa, 38 kDa, 41 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC026101 |
| Gene Symbol | NDEL1 |
| Gene ID (NCBI) | 81565 |
| RRID | AB_3670456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9GZM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDEL1 antibody 68954-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |