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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2C2

Cat no : CL488-66401

Print datasheet

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

验证数据展示

  • IF Staining of Jurkat using CL488-66401

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

  • IF Staining of Jurkat using CL488-66401

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66401-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66401 targets NDP52 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2919321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NDP52 antibody CL488-66401Download protocol
Standard Protocols
Click here to view our Standard Protocols