验证数据展示
经过测试的应用
Positive IF/ICC detected in | Jurkat cells |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66401 targets NDP52 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | NDP52 fusion protein Ag2866 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | calcium binding and coiled-coil domain 2 |
别名 | CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2 |
计算分子量 | 446 aa, 52 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC015893 |
基因名称 | NDP52 |
Gene ID (NCBI) | 10241 |
RRID | AB_2919986 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13137 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,
实验方案
Product Specific Protocols | |
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IF protocol for CL594 NDP52 antibody CL594-66401 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |