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验证数据展示

  • IF Staining of HepG2 using CL488-68253

    IF Staining of HepG2 using CL488-68253

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 NDUFS1 antibody (CL488-68253, Clone: 3H5E8 ) at dilution of 1:100.

  • FC experiment of Jurkat using CL488-68253

    FC experiment of Jurkat using CL488-68253

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NDUFS1 (CL488-68253, Clone:3H5E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated NDUFS1 Monoclonal antibody

NDUFS1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68253

产品说明书

CloneNo. 3H5E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC, FC (Intra)

别名

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68253-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68253 targets NDUFS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 NDUFS1 fusion protein Ag3135 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
别名 EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd
计算分子量 727 aa, 79 kDa
观测分子量68-81 kDa
GenBank蛋白编号BC030833
基因名称 NDUFS1
Gene ID (NCBI) 4719
RRIDAB_3084457
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP28331
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NDUFS1 antibody CL488-68253Download protocol
Standard Protocols
Click here to view our Standard Protocols