NDUFS2 Recombinant antibody
NDUFS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
242238D4
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive FC (Intra) detected in | HepG2 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84880-1-RR targets NDUFS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | NDUFS2 fusion protein Ag28008 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) |
| Synonyms | EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD, CI 49kD |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC000170 |
| Gene Symbol | NDUFS2 |
| Gene ID (NCBI) | 4720 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFS2 antibody 84880-1-RR | Download protocol |
| FC protocol for NDUFS2 antibody 84880-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
