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NDUFS2 Recombinant antibody, PBS Only (Detector)

NDUFS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242238D4

Cat no : 84880-1-PBS

Print datasheet

Synonyms

EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD, CI 49kD

验证数据展示

  • WB analysis using 84880-1-RR (same clone as 84880-1-PBS)

    WB analysis using 84880-1-RR (same clone as 84880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84880-1-RR (NDUFS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84880-1-RR (same clone as 84880-1-PBS)

    FC experiment of HepG2 using 84880-1-RR (same clone as 84880-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84880-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01653-2

    Cytometric bead array standard curve of MP01653-2

    Cytometric bead array standard curve of MP01653-2, NDUFS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84880-3-PBS. Detection antibody: 84880-1-PBS. Standard: Ag28008. Range: 0.938-60 ng/mL.

产品信息

84880-1-PBS targets NDUFS2 as part of a matched antibody pair:

MP01653-2: 84880-3-PBS capture and 84880-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDUFS2 fusion protein Ag28008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Synonyms EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD, CI 49kD
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC000170
Gene Symbol NDUFS2
Gene ID (NCBI) 4720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75306
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).