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NEDD4 Recombinant antibody, PBS Only (Capture/Detector)

NEDD4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230478A1

Cat no : 83112-2-PBS

Print datasheet

Synonyms

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

验证数据展示

  • Sandwich ELISA standard curve of MP00234-2

    Sandwich ELISA standard curve of MP00234-2

    Sandwich ELISA standard curve of MP00234-2, Human NEDD4 Recombinant Matched Antibody Pair - PBS only. 83112-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16291. 83112-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00234-2

    Sample test of MP00234-2

    The mean NEDD4 concentration was determined to be 14.63 ng/mL in C6 cell extract based on a 2.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1, NEDD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83112-2-PBS. Detection antibody: 83112-4-PBS. Standard: Ag16291. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83112-2-PBS

    Affinity and Kinetic Characterization of 83112-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83112-2-PBS against Human NEDD4 was performed. The affinity constant is 4.26 nM.

产品信息

83112-2-PBS targets NEDD4 as part of a matched antibody pair:

MP00234-1: 83112-2-PBS capture and 83112-4-PBS detection (validated in Cytometric bead array)

MP00234-2: 83112-5-PBS capture and 83112-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen NEDD4 fusion protein Ag16291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4
Synonyms KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein
Calculated Molecular Weight 1319 aa, 149 kDa
GenBank Accession NumberBC152452
Gene Symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46934
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C