CoraLite® Plus 750-conjugated NEDD4 Recombinant antibody

NEDD4 Uni-rAbTM Recombinant Antibody for WB
Cat No. CL750-83112

产品说明书

CloneNo. 230478F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inC6 cells
For other applications, we recommend the unconjugated version of this antibody, 83112-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83112 targets NEDD4 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 NEDD4 fusion protein Ag16291 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4
别名
计算分子量 1319 aa, 149 kDa
观测分子量 115 kDa
GenBank蛋白编号BC152452
基因名称 NEDD4
Gene ID (NCBI) 4734
RRIDAB_3673770
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46934
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 NEDD4 antibody CL750-83112Download protocol
Standard Protocols
Click here to view our Standard Protocols
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