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验证数据展示

  • IF Staining of mouse brain using CL488-28180

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-28180

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using CL488-28180

    FC experiment of Jurkat using CL488-28180

    1x10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NET1 (CL488-28180)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NET1 Polyclonal antibody

NET1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-28180

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF-P, FC (Intra)

Synonyms

ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy NET1 Ready-To-Use IHC Kit. NET1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28180-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28180 targets NET1 in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, Mouse
Immunogen NET1 fusion protein Ag28142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroepithelial cell transforming 1
Synonyms ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1
Calculated Molecular Weight 596 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC010285
Gene Symbol NET1
Gene ID (NCBI) 10276
RRIDAB_3672824
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z628
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
FC protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
Standard Protocols
Click here to view our Standard Protocols