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验证数据展示

  • WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

    WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

    WB result of 18934-1-AP. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-H/NF200 antibody included
  • IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-H/NF200 antibody included
  • IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

    IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

NF-H/NF200 Polyclonal antibody, PBS Only

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 18934-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NF-H/NF200 Ready-To-Use IHC Kit. NF-H/NF200 primary antibody included.

产品信息

18934-1-PBS targets NF-H/NF200 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NF-H/NF200 fusion protein Ag13517 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofilament, heavy polypeptide
别名 NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain
计算分子量 112 kDa
观测分子量 200 kDa, 140-160 kDa
GenBank蛋白编号BC014185
基因名称 NF-H
Gene ID (NCBI) 4744
RRIDAB_10640801
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12036
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.