• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

    WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66917-1-Ig (NFATC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

    FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 (66917-1-Ig, Clone:1F3B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFATC2 antibody included

NFATC2 Monoclonal antibody, PBS Only

NFATC2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66917-1-PBS

产品说明书

CloneNo. 1F3B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy NFATC2 Ready-To-Use IHC Kit. NFATC2 primary antibody included.

产品信息

66917-1-PBS targets NFATC2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NFATC2 fusion protein Ag17990 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
别名 NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp
计算分子量 925 aa, 100 kDa
观测分子量 135-140 kDa
GenBank蛋白编号BC136418
基因名称 NFATC2
Gene ID (NCBI) 4773
RRIDAB_2882244
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13469
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.