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NFATC2 Monoclonal antibody, PBS Only

NFATC2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3B2

Cat no : 66917-1-PBS

Print datasheet

Synonyms

NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp

验证数据展示

  • WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

    WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66917-1-Ig (NFATC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

    FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 (66917-1-Ig, Clone:1F3B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFATC2 antibody included
Planning an IHC experiment? We recommend our IHCeasy NFATC2 Ready-To-Use IHC Kit. NFATC2 primary antibody included.

产品信息

66917-1-PBS targets NFATC2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen NFATC2 fusion protein Ag17990 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
Synonyms NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp
Calculated Molecular Weight 925 aa, 100 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberBC136418
Gene Symbol NFATC2
Gene ID (NCBI) 4773
RRIDAB_2882244
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13469
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.