NFATC2 Monoclonal antibody
NFATC2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1F3B2
验证数据展示
经过测试的应用
Positive WB detected in | Ramos cells, Daudi cells, Jurkat cells |
Positive IHC detected in | human breast cancer tissue, human lymphoma tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66917-1-Ig targets NFATC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | NFATC2 fusion protein Ag17990 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 |
Synonyms | NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp |
Calculated Molecular Weight | 925 aa, 100 kDa |
Observed Molecular Weight | 135-140 kDa |
GenBank Accession Number | BC136418 |
Gene Symbol | NFATC2 |
Gene ID (NCBI) | 4773 |
RRID | AB_2882244 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13469 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NFATC2 antibody 66917-1-Ig | Download protocol |
IHC protocol for NFATC2 antibody 66917-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |