CoraLite®594-conjugated NFATC2 Monoclonal antibody

NFATC2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66917

产品说明书

CloneNo. 1F3B2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NFATC2 Ready-To-Use IHC Kit. NFATC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66917-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66917 targets NFATC2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 NFATC2 fusion protein Ag17990 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
别名 NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp
计算分子量 925 aa, 100 kDa
观测分子量 135-140 kDa
GenBank蛋白编号BC136418
基因名称 NFATC2
Gene ID (NCBI) 4773
RRIDAB_3084736
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13469
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 NFATC2 antibody CL594-66917Download protocol
Standard Protocols
Click here to view our Standard Protocols
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