验证数据展示
经过测试的应用
Positive WB detected in | HSC-T6 cells, Saos-2 cells, U2OS cellls, LNCaP cells, HeLa cells, NIH/3T3 cells, 4T1 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68318-1-Ig targets NFIC in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | NFIC fusion protein Ag9472 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | nuclear factor I/C (CCAAT-binding transcription factor) |
别名 | NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor |
计算分子量 | 428aa,48 kDa; 508aa,57 kDa |
观测分子量 | 50-56 kDa |
GenBank蛋白编号 | BC012120 |
基因名称 | NFIC |
Gene ID (NCBI) | 4782 |
RRID | AB_3085054 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08651 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
实验方案
Product Specific Protocols | |
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WB protocol for NFIC antibody 68318-1-Ig | Download protocol |
FC protocol for NFIC antibody 68318-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |