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NFIC Monoclonal antibody

NFIC Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A11A5

Cat no : 68318-1-Ig

Print datasheet

Synonyms

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

验证数据展示

  • WB analysis using 68318-1-Ig

    WB analysis using 68318-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HeLa using 68318-1-Ig

    FC experiment of HeLa using 68318-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHSC-T6 cells, Saos-2 cells, U2OS cellls, LNCaP cells, HeLa cells, NIH/3T3 cells, 4T1 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFIC Ready-To-Use IHC Kit. NFIC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68318-1-Ig targets NFIC in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NFIC fusion protein Ag9472 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear factor I/C (CCAAT-binding transcription factor)
Synonyms NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor
Calculated Molecular Weight428aa,48 kDa; 508aa,57 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC012120
Gene Symbol NFIC
Gene ID (NCBI) 4782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

实验方案

Product Specific Protocols
WB protocol for NFIC antibody 68318-1-IgDownload protocol
FC protocol for NFIC antibody 68318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols