• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NFIC Monoclonal antibody, PBS Only

NFIC Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11A5

Cat no : 68318-1-PBS

Print datasheet

Synonyms

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

验证数据展示

  • WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

    FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NFIC Ready-To-Use IHC Kit. NFIC primary antibody included.

产品信息

68318-1-PBS targets NFIC in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NFIC fusion protein Ag9472 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear factor I/C (CCAAT-binding transcription factor)
Synonyms NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor
Calculated Molecular Weight428aa,48 kDa; 508aa,57 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC012120
Gene Symbol NFIC
Gene ID (NCBI) 4782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08651
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.