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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

    WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

    IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NME3 antibody (84278-5-RR, Clone: 241585H7 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01200-2

    Cytometric bead array standard curve of MP01200-2

    Cytometric bead array standard curve of MP01200-2, NME3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84278-5-PBS. Detection antibody: 84278-1-PBS. Standard: Ag7284. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84278-5-RR

    Affinity and Kinetic Characterization of 84278-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84278-5-RR against Human NME3 were performed. The affinity constant is below 1 pM.

NME3 Recombinant antibody, PBS Only (Capture)

NME3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84278-5-PBS
CloneNo. 241585H7

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23, DR nm23

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84278-5-PBS targets NME3 as part of a matched antibody pair:

MP01200-2: 84278-5-PBS capture and 84278-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen NME3 fusion protein Ag7284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name non-metastatic cells 3, protein expressed in
Synonyms NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23, DR nm23
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC000250
Gene Symbol NME3
Gene ID (NCBI) 4832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13232
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)