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NME3 Recombinant antibody

NME3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241585H7

Cat no : 84278-5-RR

Print datasheet

Synonyms

NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23, DR nm23

验证数据展示

  • WB analysis using 84278-5-RR

    WB analysis using 84278-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 84278-5-RR

    IHC staining of rat kidney using 84278-5-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 84278-5-RR

    IHC staining of rat kidney using 84278-5-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84278-5-RR

    IF Staining of HeLa using 84278-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NME3 antibody (84278-5-RR, Clone: 241585H7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84278-5-RR

    Affinity and Kinetic Characterization of 84278-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84278-5-RR against Human NME3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, K-562 cells
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84278-5-RR is NME3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen NME3 fusion protein Ag7284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name non-metastatic cells 3, protein expressed in
Synonyms NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23, DR nm23
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC000250
Gene Symbol NME3
Gene ID (NCBI) 4832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)

实验方案

Product Specific Protocols
WB protocol for NME3 antibody 84278-5-RRDownload protocol
IHC protocol for NME3 antibody 84278-5-RRDownload protocol
IF protocol for NME3 antibody 84278-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols