验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 84278-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84278-5 targets NME3 in IF/ICC applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, rat |
| Immunogen | NME3 fusion protein Ag7284 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | non-metastatic cells 3, protein expressed in |
| Synonyms | |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC000250 |
| Gene Symbol | NME3 |
| Gene ID (NCBI) | 4832 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13232 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NME3 antibody CL488-84278-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |