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NPTX2 Monoclonal antibody

NPTX2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H8D8

Cat no : 68709-1-Ig

Print datasheet

Synonyms

NP-II, NP2, Neuronal pentraxin-2, Neuronal pentraxin II, 2H8D8

验证数据展示

  • WB analysis using 68709-1-Ig

    WB analysis using 68709-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68709-1-Ig (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68709-1-Ig

    IF Staining of SH-SY5Y using 68709-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NPTX2 antibody (68709-1-Ig, Clone: 2H8D8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inpig pituitary gland tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68709-1-Ig targets NPTX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen NPTX2 fusion protein Ag33808 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neuronal pentraxin II
Synonyms NP-II, NP2, Neuronal pentraxin-2, Neuronal pentraxin II, 2H8D8
Calculated Molecular Weight 47 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC009924
Gene Symbol NPTX2
Gene ID (NCBI) 4885
RRIDAB_3670402
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47972
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

实验方案

Product Specific Protocols
WB protocol for NPTX2 antibody 68709-1-IgDownload protocol
IF protocol for NPTX2 antibody 68709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols