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CoraLite® Plus 488-conjugated NRF1/nuclear respiratory factor 1 Monoclonal antibody

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C2B8

Cat no : CL488-66832

Print datasheet

Synonyms

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

验证数据展示

  • IF Staining of HepG2 using CL488-66832

    IF Staining of HepG2 using CL488-66832

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NRF1 antibody (CL488-66832, Clone: 3C2B8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66832

    FC experiment of HepG2 using CL488-66832

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NRF1/nuclear respiratory factor 1 (CL488-66832, Clone:3C2B8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66832-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66832 targets NRF1/nuclear respiratory factor 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear respiratory factor 1
Synonyms NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2883385
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NRF1/nuclear respiratory factor 1 antibody CL488-66832Download protocol
FC protocol for CL Plus 488 NRF1/nuclear respiratory factor 1 antibody CL488-66832Download protocol
Standard Protocols
Click here to view our Standard Protocols