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验证数据展示

  • IF Staining of HepG2 using CL594-66832

    IF Staining of HepG2 using CL594-66832

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66832 (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66832

    FC experiment of HepG2 using CL594-66832

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NRF1/nuclear respiratory factor 1 (CL594-66832, Clone:3C2B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated NRF1/nuclear respiratory factor 1 Monoclonal antibody

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66832

产品说明书

CloneNo. 3C2B8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66832-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66832 targets NRF1/nuclear respiratory factor 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 NRF1/nuclear respiratory factor 1 fusion protein Ag28149 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear respiratory factor 1
别名 NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein
计算分子量 522 aa, 56 kDa
GenBank蛋白编号BC016925
基因名称 NRF1
Gene ID (NCBI) 4899
RRIDAB_2883629
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16656
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 NRF1/nuclear respiratory factor 1 antibody CL594-66832Download protocol
Standard Protocols
Click here to view our Standard Protocols