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NRG1, isoform SMDF Recombinant antibody, PBS Only

NRG1, isoform SMDF Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240184G2

Cat no : 83323-6-PBS

Print datasheet

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

验证数据展示

  • IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83323-6-RR

    Affinity and Kinetic Characterization of 83323-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

产品信息

83323-6-PBS targets NRG1, isoform SMDF in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neuregulin 1
Synonyms NRG1, NRG1A, Heregulin, Glial growth factor, GGF2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ02297
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.