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验证数据展示

  • IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

    IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83323-6-RR

    Affinity and Kinetic Characterization of 83323-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

NRG1, isoform SMDF Recombinant antibody, PBS Only

NRG1, isoform SMDF Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 83323-6-PBS

产品说明书

CloneNo. 240184G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IF-P, ELISA

别名

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83323-6-PBS targets NRG1, isoform SMDF in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuregulin 1
别名 NRG1, NRG1A, Heregulin, Glial growth factor, GGF2
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007675
基因名称 NRG1
Gene ID (NCBI) 3084
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ02297
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.