验证数据展示
经过测试的应用
Positive IHC detected in | mouse cerebellum tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:125-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83323-6-RR targets NRG1, isoform SMDF in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IF/ICC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | NRG1, isoform SMDF fusion protein Ag0803 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | neuregulin 1 |
Synonyms | NRG1, NRG1A, Heregulin, Glial growth factor, GGF2 |
Calculated Molecular Weight | 70 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC007675 |
Gene Symbol | NRG1 |
Gene ID (NCBI) | 3084 |
RRID | AB_3670989 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q02297 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.
实验方案
Product Specific Protocols | |
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IHC protocol for NRG1, isoform SMDF antibody 83323-6-RR | Download protocol |
IF protocol for NRG1, isoform SMDF antibody 83323-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |