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NSD3 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 19.5-625 pg/mL

Cat No : MP00189-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

    Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

    Sample test of MP00189-4

    The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00189-4 targets NSD3 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 19.5-625 pg/mL (Sandwich ELISA);

Capture antibody: NSD3 Recombinant antibody, PBS Only (Capture) 83116-2-PBS (230499C2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: WHSC1L1 Recombinant antibody, PBS Only (Detector) 83116-5-PBS (230499C4). 100 μg. Concentration 1 mgl/ml.

Alternative NSD3 matched antibody pairs: MP00189-1, MP00189-2, MP00189-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83116-2-PBS
Tested Reactivity human
Immunogen NSD3 fusion protein Ag1894
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Synonyms WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3
GenBank accession numberBC012059
Gene symbol WHSC1L1
UniprotQ9BZ95
Gene ID (NCBI) 54904
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83116-5-PBS
Tested Reactivity human
Immunogen WHSC1L1 fusion protein Ag1894
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Synonyms NSD3, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3, EC:2.1.1.370
GenBank accession numberBC012059
Gene symbol WHSC1L1
UniprotQ9BZ95
Gene ID (NCBI) 54904
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C