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CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Polyclonal antibody

NUCB2/nesfatin-1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26712

Print datasheet

Synonyms

DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, NUCB2, NUCB2/nesfatin-1, nucleobindin 2

验证数据展示

  • IF Staining of HepG2 using CL488-26712

    IF Staining of HepG2 using CL488-26712

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NUCB2/nesfatin-1 antibody (CL488-26712) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-26712

    FC experiment of HepG2 using CL488-26712

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Polyclonal antibody (CL488-26712)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 26712-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26712 targets NUCB2/nesfatin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleobindin 2
Synonyms DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, NUCB2, NUCB2/nesfatin-1, nucleobindin 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_005013
Gene Symbol NUCB2
Gene ID (NCBI) 4925
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUCB2/nesfatin-1 antibody CL488-26712Download protocol
FC protocol for CL Plus 488 NUCB2/nesfatin-1 antibody CL488-26712Download protocol
Standard Protocols
Click here to view our Standard Protocols