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验证数据展示

  • IF Staining of HepG2 using CL488-26712

    IF Staining of HepG2 using CL488-26712

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NUCB2/nesfatin-1 antibody (CL488-26712) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-26712

    FC experiment of HepG2 using CL488-26712

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Polyclonal antibody (CL488-26712)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Polyclonal antibody

NUCB2/nesfatin-1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26712

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 26712-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26712 targets NUCB2/nesfatin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleobindin 2
别名 NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4
计算分子量 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_005013
基因名称 NUCB2
Gene ID (NCBI) 4925
RRIDAB_3672802
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUCB2/nesfatin-1 antibody CL488-26712Download protocol
FC protocol for CL Plus 488 NUCB2/nesfatin-1 antibody CL488-26712Download protocol
Standard Protocols
Click here to view our Standard Protocols