验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | NVL fusion protein Ag9321 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | nuclear VCP-like |
别名 | NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like |
计算分子量 | 856aa,95 kDa; 659aa,73 kDa |
观测分子量 | 95 kDa |
GenBank蛋白编号 | BC012105 |
基因名称 | NVL |
Gene ID (NCBI) | 4931 |
RRID | AB_3085116 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O15381 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for NVL antibody 68399-1-Ig | Download protocol |
FC protocol for NVL antibody 68399-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |