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NVL Monoclonal antibody

NVL Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D8G4

Cat no : 68399-1-Ig

Print datasheet

Synonyms

NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like

验证数据展示

  • WB analysis using 68399-1-Ig

    WB analysis using 68399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 68399-1-Ig

    FC experiment of HeLa using 68399-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NVL fusion protein Ag9321 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear VCP-like
Synonyms NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like
Calculated Molecular Weight856aa,95 kDa; 659aa,73 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC012105
Gene Symbol NVL
Gene ID (NCBI) 4931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NVL antibody 68399-1-IgDownload protocol
FC protocol for NVL antibody 68399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols