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验证数据展示

  • WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-PBS

    FC experiment of HeLa using 68399-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NVL Monoclonal antibody, PBS Only (68399-1-PBS, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NVL Monoclonal antibody, PBS Only

NVL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68399-1-PBS

产品说明书

CloneNo. 1D8G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68399-1-PBS targets NVL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 NVL fusion protein Ag9321 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear VCP-like
别名 NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like
计算分子量856aa,95 kDa; 659aa,73 kDa
观测分子量 95 kDa
GenBank蛋白编号BC012105
基因名称 NVL
Gene ID (NCBI) 4931
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15381
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for NVL antibody 68399-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols