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NVL Monoclonal antibody, PBS Only

NVL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8G4

Cat no : 68399-1-PBS

Print datasheet

Synonyms

NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like

验证数据展示

  • WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68399-1-PBS

    FC experiment of HeLa using 68399-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NVL Monoclonal antibody, PBS Only (68399-1-PBS, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68399-1-PBS targets NVL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen NVL fusion protein Ag9321 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear VCP-like
Synonyms NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like
Calculated Molecular Weight856aa,95 kDa; 659aa,73 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC012105
Gene Symbol NVL
Gene ID (NCBI) 4931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15381
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for NVL antibody 68399-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols