CoraLite® Plus 488-conjugated NXN Monoclonal antibody
NXN Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C6A4
验证数据展示
经过测试的应用
| Positive IF detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66603-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66603 targets NXN in IF applications and shows reactivity with Human, Mouse , Rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse , Rat |
| Immunogen | NXN fusion protein Ag9248 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nucleoredoxin |
| Synonyms | FLJ12614, NRX, nucleoredoxin, NXN, TRG 4 |
| Calculated Molecular Weight | 435 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC009327 |
| Gene Symbol | NXN |
| Gene ID (NCBI) | 64359 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q6DKJ4 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NXN antibody CL488-66603 | Download protocol |
| FC protocol for CL Plus 488 NXN antibody CL488-66603 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
