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验证数据展示

  • IF Staining of HEK-293 using CL594-66603

    IF Staining of HEK-293 using CL594-66603

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 NXN antibody (CL594-66603, Clone: 2C6A4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66603

    FC experiment of HeLa using CL594-66603

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NXN (CL594-66603, Clone:2C6A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated NXN Monoclonal antibody

NXN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66603

产品说明书

CloneNo. 2C6A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

TRG 4, Nucleoredoxin, NRX, EC:1.8.1.8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66603-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66603 targets NXN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NXN fusion protein Ag9248 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nucleoredoxin
别名 TRG 4, Nucleoredoxin, NRX, EC:1.8.1.8
计算分子量 435 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009327
基因名称 NXN
Gene ID (NCBI) 64359
RRIDAB_2934735
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6DKJ4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

实验方案

Product Specific Protocols
IF protocol for CL594 NXN antibody CL594-66603Download protocol
Standard Protocols
Click here to view our Standard Protocols