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Nectin-2/CD112 Polyclonal antibody

Nectin-2/CD112 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27171-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

Nectin-2, Nectin cell adhesion molecule 2, Nectin 2, HVEB, Herpesvirus entry mediator B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat kidney tissue, HAP1 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Nectin-2/CD112 Ready-To-Use IHC Kit. Nectin-2/CD112 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27171-1-AP targets Nectin-2/CD112 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Nectin-2/CD112 fusion protein Ag26040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poliovirus receptor-related 2 (herpesvirus entry mediator B)
别名 Nectin-2, Nectin cell adhesion molecule 2, Nectin 2, HVEB, Herpesvirus entry mediator B
计算分子量 58 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC003091
基因名称 Nectin-2/CD112
Gene ID (NCBI) 5819
RRIDAB_2880784
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

实验方案

Product Specific Protocols
WB protocol for Nectin-2/CD112 antibody 27171-1-APDownload protocol
IHC protocol for Nectin-2/CD112 antibody 27171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Mucosal Immunol

Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.

Authors - Changying Wang
ratIF

J Transl Int Med

Lipid-related protein NECTIN2 is an important marker in the progression of carotid atherosclerosis: An intersection of clinical and basic studies.

Authors - Shen Li
humanIHC

iScience

Proteomic and single-cell landscape reveals novel pathogenic mechanisms of HBV-infected intrahepatic cholangiocarcinoma

Authors - Yifei Shen
humanIF

Sci Rep

Single-cell RNA sequencing unveils tumor heterogeneity and immune microenvironment between subungual and plantar melanoma

Authors - Panpan Wang
humanIF

Cancer Lett

Histone Lactylation Dynamics: Unlocking the Triad of Metabolism, Epigenetics, and Immune Regulation in Metastatic Cascade of Pancreatic Cancer

Authors - Xing Wang
mouseWB

Neurosci Bull

Dorsal CA1 NECTIN3 Reduction Mediates Early-Life Stress-Induced Object Recognition Memory Deficits in Adolescent Female Mice

Authors - Yu-Nu Ma
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