CoraLite® Plus 488-conjugated ORM1/2 Monoclonal antibody
ORM1/2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5F2H7
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells, mouse liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66097-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66097 targets ORM1/2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | ORM1/2 fusion protein Ag19248 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | orosomucoid 1 |
| Synonyms | AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1 |
| Calculated Molecular Weight | 201 aa, 24 kDa |
| Observed Molecular Weight | 40-47 kDa |
| GenBank Accession Number | BC026238 |
| Gene Symbol | ORM1 |
| Gene ID (NCBI) | 5004 |
| RRID | AB_2883258 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02763 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ORM1/2 antibody CL488-66097 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
