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验证数据展示

  • IF Staining of HepG2 using CL594-66097

    IF Staining of HepG2 using CL594-66097

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66097 (ORM1/2 antibody) at dilution of 1:50.

  • FC experiment of HepG2 using CL594-66097

    FC experiment of HepG2 using CL594-66097

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ORM1/2 (CL594-66097, Clone:5F2H7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated ORM1/2 Monoclonal antibody

ORM1/2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66097

产品说明书

CloneNo. 5F2H7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66097-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66097 targets ORM1/2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 ORM1/2 fusion protein Ag19248 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 orosomucoid 1
别名 AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1
计算分子量 201 aa, 24 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC026238
基因名称 ORM1
Gene ID (NCBI) 5004
RRIDAB_2883511
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02763
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

实验方案

Product Specific Protocols
IF protocol for CL594 ORM1/2 antibody CL594-66097Download protocol
Standard Protocols
Click here to view our Standard Protocols