验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HUVEC cells, U2OS cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-82932 targets OS9 in WB, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | WB, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | OS9 fusion protein Ag0106 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | osteosarcoma amplified 9, endoplasmic reticulum associated protein |
Synonyms | Protein OS-9, Amplified in osteosarcoma 9, 230149B4 |
Calculated Molecular Weight | 76 kDa |
Observed Molecular Weight | 83-97 kDa |
GenBank Accession Number | BC000532 |
Gene Symbol | OS9 |
Gene ID (NCBI) | 10956 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13438 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 OS9 antibody CL750-82932 | Download protocol |
FC protocol for CL Plus 750 OS9 antibody CL750-82932 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |