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验证数据展示

  • FC experiment of HeLa using CL750-82932

    FC experiment of HeLa using CL750-82932

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 750 Os9 Recombinant Antibody (CL750-82932, Clone:230149B4)(red), or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL750-82932

    WB analysis using CL750-82932

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82932 (OS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated OS9 Recombinant antibody

OS9 Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82932
CloneNo. 230149B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, U2OS cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82932-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82932 targets OS9 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen OS9 fusion protein Ag0106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Synonyms Protein OS-9, Amplified in osteosarcoma 9, 230149B4
Calculated Molecular Weight 76 kDa
Observed Molecular Weight83-97 kDa
GenBank Accession NumberBC000532
Gene Symbol OS9
Gene ID (NCBI) 10956
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13438
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 OS9 antibody CL750-82932Download protocol
FC protocol for CL Plus 750 OS9 antibody CL750-82932Download protocol
Standard Protocols
Click here to view our Standard Protocols