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验证数据展示

  • FC experiment of HeLa using CL750-82932

    FC experiment of HeLa using CL750-82932

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 750 Os9 Recombinant Antibody (CL750-82932, Clone:230149B4)(red), or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL750-82932

    WB analysis using CL750-82932

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82932 (OS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated OS9 Recombinant antibody

OS9 Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82932

产品说明书

CloneNo. 230149B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra)

别名

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, U2OS cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82932-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82932 targets OS9 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 OS9 fusion protein Ag0106 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 osteosarcoma amplified 9, endoplasmic reticulum associated protein
别名 Protein OS-9, Amplified in osteosarcoma 9, 230149B4
计算分子量 76 kDa
观测分子量83-97 kDa
GenBank蛋白编号BC000532
基因名称 OS9
Gene ID (NCBI) 10956
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13438
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 OS9 antibody CL750-82932Download protocol
FC protocol for CL Plus 750 OS9 antibody CL750-82932Download protocol
Standard Protocols
Click here to view our Standard Protocols