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- KD/KO Validated
PARK7/DJ-1 Recombinant antibody
PARK7/DJ-1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
230124B7
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, BxPC-3 cells, Jurkat cells, HSC-T6 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82913-2-RR targets PARK7/DJ-1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | PARK7/DJ-1 fusion protein Ag2287 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | Parkinson disease (autosomal recessive, early onset) 7 |
Synonyms | PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1 |
Calculated Molecular Weight | 189 aa, 20 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC008188 |
Gene Symbol | PARK7 |
Gene ID (NCBI) | 11315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q99497 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PARK7/DJ-1 antibody 82913-2-RR | Download protocol |
IF protocol for PARK7/DJ-1 antibody 82913-2-RR | Download protocol |
IP protocol for PARK7/DJ-1 antibody 82913-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |