CoraLite® Plus 488-conjugated PARK7/DJ-1 Recombinant antibody
PARK7/DJ-1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
230124B7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PARK7 Ready-To-Use IHC Kit. PARK7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82913-2-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82913-2 targets PARK7/DJ-1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PARK7/DJ-1 fusion protein Ag2287 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | Parkinson disease (autosomal recessive, early onset) 7 |
| Synonyms | |
| Calculated Molecular Weight | 189 aa, 20 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC008188 |
| Gene Symbol | PARK7 |
| Gene ID (NCBI) | 11315 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99497 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PARK7/DJ-1 antibody CL488-82913-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |