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PARP1 Polyclonal antibody
PARP1 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
FC (Intra), IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
563
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human liver cancer tissue, mouse testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells, mouse testis tissue, MCF-7 cells |
Positive FC detected in | K-562 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13371-1-AP targets PARP1 in WB, IP, IHC, IF, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra), IF, IHC, IP, WB, ELISA |
Cited Applications | ChIP, CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep, monkey, pig, canine, bovine, Fungus |
Immunogen | PARP1 fusion protein Ag4193 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | poly (ADP-ribose) polymerase 1 |
Synonyms | ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL |
Calculated Molecular Weight | 1014 aa, 113 kDa |
Observed Molecular Weight | 113-116 kDa, 89 kDa |
GenBank Accession Number | BC037545 |
Gene Symbol | PARP1 |
Gene ID (NCBI) | 142 |
RRID | AB_2160459 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P09874 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PARP1 antibody 13371-1-AP | Download protocol |
IHC protocol for PARP1 antibody 13371-1-AP | Download protocol |
IF protocol for PARP1 antibody 13371-1-AP | Download protocol |
IP protocol for PARP1 antibody 13371-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. | ||
Exp Mol Med Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury | ||
Nat Commun The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. | ||