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PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13371-1-AP

Print datasheet

Synonyms

DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

验证数据展示

  • WB analysis using 13371-1-AP

    WB analysis using 13371-1-AP

    Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 13371-1-AP

    IHC staining of mouse colon using 13371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • FC experiment of K-562 using 13371-1-AP

    FC experiment of K-562 using 13371-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PARP1 (13371-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using 13371-1-AP

    WB analysis using 13371-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13371-1-AP

    WB analysis using 13371-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13371-1-AP

    WB analysis of HeLa using 13371-1-AP

    Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IP experiment of K-562 using 13371-1-AP

    IP experiment of K-562 using 13371-1-AP

    IP result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.

  • IF Staining of mouse testis using 13371-1-AP

    IF Staining of mouse testis using 13371-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 13371-1-AP

    IF Staining of MCF-7 using 13371-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 13371-1-AP

    IHC staining of mouse testis using 13371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of human lung cancer using 13371-1-AP

    IHC staining of human lung cancer using 13371-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of mouse testis using 13371-1-AP

    IHC staining of mouse testis using 13371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of mouse colon using 13371-1-AP

    IHC staining of mouse colon using 13371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP1 antibody included
  • WB analysis of HeLa using 13371-1-AP

    WB analysis of HeLa using 13371-1-AP

    Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 13371-1-AP

    IF Staining of HEK-293 using 13371-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inHEK-293 cells, MCF-7 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13371-1-AP targets PARP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, chicken, bovine, sheep, fungus
Immunogen PARP1 fusion protein Ag4193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2160459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

实验方案

Product Specific Protocols
WB protocol for PARP1 antibody 13371-1-APDownload protocol
IHC protocol for PARP1 antibody 13371-1-APDownload protocol
IF protocol for PARP1 antibody 13371-1-APDownload protocol
IP protocol for PARP1 antibody 13371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
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humanWB

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li
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humanWB

ACS Nano

PMID: 31670945

Authors - Ying Xu
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mouseWB

Exp Mol Med

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Authors - Zhenjun Yu
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humanWB

Nat Commun

The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.

Authors - Guangjian Fan
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humanWB

Nat Commun

Loss of KLF14 triggers centrosome amplification and tumorigenesis.

Authors - Guangjian Fan
View Article