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验证数据展示

  • IF Staining of HepG2 using CL488-13371

    IF Staining of HepG2 using CL488-13371

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PARP1 antibody (CL488-13371) at dilution of 1:200.

  • FC experiment of K-562 using CL488-13371

    FC experiment of K-562 using CL488-13371

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human PARP1 (CL488-13371) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-13371

    IF Staining of HepG2 using CL488-13371

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PARP1 antibody (CL488-13371) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13371

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13371-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13371 targets PARP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PARP1 fusion protein Ag4193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 1
别名 DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT
计算分子量 1014 aa, 113 kDa
观测分子量 113-116 kDa, 89 kDa
GenBank蛋白编号BC037545
基因名称 PARP1
Gene ID (NCBI) 142
RRIDAB_2919070
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09874
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PARP1 antibody CL488-13371Download protocol
Standard Protocols
Click here to view our Standard Protocols