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  • Featured Product
  • KD/KO Validated

PARP1 Monoclonal antibody, PBS Only (Capture/Detector)

PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1D7D4

Cat no : 66520-1-PBS

Print datasheet

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

验证数据展示

  • WB analysis of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

    WB analysis of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

    Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66520-1-Ig (same clone as 66520-1-PBS)

    WB analysis of HSC-T6 using 66520-1-Ig (same clone as 66520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 66520-1-Ig (same clone as 66520-1-PBS)

    IP experiment of K-562 using 66520-1-Ig (same clone as 66520-1-PBS)

    IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 66520-1-Ig (same clone as 66520-1-PBS)

    IHC staining of rat colon using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IHC staining of mouse testis using 66520-1-Ig (same clone as 66520-1-PBS)

    IHC staining of mouse testis using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • FC experiment of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

    FC experiment of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66520-1-Ig (same clone as 66520-1-PBS)

    IHC staining of mouse colon using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

    WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Sample test of MP50985-1

    Sample test of MP50985-1

    Cytometric bead array in cell lysate using MP50985-1, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66520-1-PBS. Detection antibody: 60555-2-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

  • Sample test of MP50985-2

    Sample test of MP50985-2

    Cytometric bead array in cell lysate using MP50985-2, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-1-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

  • IF Staining of Neuro-2a using 66520-1-Ig (same clone as 66520-1-PBS)

    IF Staining of Neuro-2a using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.

产品信息

66520-1-PBS targets PARP1 as part of a matched antibody pair:

MP50985-1: 66520-1-PBS capture and 60555-2-PBS detection (validated in N/A)

MP50985-2: 60555-1-PBS capture and 66520-1-PBS detection (validated in N/A)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen PARP1 fusion protein Ag19173 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 85-89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2881883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.