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Species Reactivity
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验证数据展示

  • WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

    WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included
  • IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included
  • IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 84345-5-RR (same clone as 84345-5-PBS)

    FC experiment of U-251 using 84345-5-RR (same clone as 84345-5-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PAX6 Recombinant antibody (84345-5-RR, Clone:241521G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

PAX6 Recombinant antibody, PBS Only

PAX6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 84345-5-PBS

产品说明书

CloneNo. 241521G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.

产品信息

84345-5-PBS targets PAX6 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PAX6 fusion protein Ag2984 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paired box 6
别名 Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC011953
基因名称 PAX6
Gene ID (NCBI) 5080
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP26367
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.