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PAX6 Recombinant antibody, PBS Only

PAX6 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241521G1

Cat no : 84345-5-PBS

Print datasheet

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

验证数据展示

  • WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

    WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included
  • IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included
  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.

产品信息

The immunogen of 84345-5-PBS is PAX6 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PAX6 fusion protein Ag2984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 6
Synonyms Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC011953
Gene Symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26367
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.