• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PAX6 Recombinant antibody

PAX6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241521G1

Cat no : 84345-5-RR

Print datasheet

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

验证数据展示

  • WB analysis using 84345-5-RR

    WB analysis using 84345-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84345-5-RR

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX6 antibody included
  • IHC staining of mouse brain using 84345-5-RR

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX6 antibody included
  • IF Staining of mouse eye using 84345-5-RR

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 84345-5-RR

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, mouse eye tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84345-5-RR targets PAX6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PAX6 fusion protein Ag2984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 6
Synonyms Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC011953
Gene Symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26367
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

实验方案

Product Specific Protocols
WB protocol for PAX6 antibody 84345-5-RRDownload protocol
IHC protocol for PAX6 antibody 84345-5-RRDownload protocol
IF protocol for PAX6 antibody 84345-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols