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验证数据展示

  • WB analysis using 84345-5-RR

    WB analysis using 84345-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84345-5-RR

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX6 antibody included
  • IHC staining of mouse brain using 84345-5-RR

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX6 antibody included
  • IF Staining of mouse eye using 84345-5-RR

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 84345-5-RR

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-251 using 84345-5-RR

    FC experiment of U-251 using 84345-5-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PAX6 Recombinant antibody (84345-5-RR, Clone:241521G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

PAX6 Recombinant antibody

PAX6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 84345-5-RR

产品说明书

CloneNo. 241521G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, mouse eye tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive FC (Intra) detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84345-5-RR targets PAX6 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 PAX6 fusion protein Ag2984 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paired box 6
别名 Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC011953
基因名称 PAX6
Gene ID (NCBI) 5080
RRIDAB_3671885
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP26367
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

实验方案

Product Specific Protocols
WB protocol for PAX6 antibody 84345-5-RRDownload protocol
IHC protocol for PAX6 antibody 84345-5-RRDownload protocol
IF protocol for PAX6 antibody 84345-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols