验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse eye tissue |
Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84345-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84345-5 targets PAX6 in IF-P applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | PAX6 fusion protein Ag2984 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | paired box 6 |
| Synonyms | |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC011953 |
| Gene Symbol | PAX6 |
| Gene ID (NCBI) | 5080 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26367 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PAX6 antibody CL488-84345-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |