验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, pig kidney tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68317-1-Ig targets PBLD in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, pig |
| Cited Reactivity | human |
| Immunogen | PBLD fusion protein Ag23827 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phenazine biosynthesis-like protein domain containing |
| Synonyms | FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC009738 |
| Gene Symbol | PBLD |
| Gene ID (NCBI) | 64081 |
| RRID | AB_3085053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P30039 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PBLD antibody 68317-1-Ig | Download protocol |
| IF protocol for PBLD antibody 68317-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |