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PBLD Monoclonal antibody

PBLD Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11D4

Cat no : 68317-1-Ig

Print datasheet

Synonyms

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

验证数据展示

  • WB analysis of pig liver using 68317-1-Ig

    WB analysis of pig liver using 68317-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig kidney using 68317-1-Ig

    WB analysis of pig kidney using 68317-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 68317-1-Ig

    IF Staining of HEK-293 using 68317-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig liver tissue, pig kidney tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68317-1-Ig targets PBLD in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig
Immunogen PBLD fusion protein Ag23827 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phenazine biosynthesis-like protein domain containing
Synonyms FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD
Observed Molecular Weight30 kDa
GenBank Accession NumberBC009738
Gene Symbol PBLD
Gene ID (NCBI) 64081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30039
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)

实验方案

Product Specific Protocols
WB protocol for PBLD antibody 68317-1-IgDownload protocol
IF protocol for PBLD antibody 68317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols