CoraLite® Plus 488-conjugated PBLD Monoclonal antibody
PBLD Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G11D4
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 68317-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68317 targets PBLD in IF/ICC applications and shows reactivity with human, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, pig |
| Immunogen | PBLD fusion protein Ag23827 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phenazine biosynthesis-like protein domain containing |
| Synonyms | MAWDBP, MAWD-binding protein, MAWD binding protein, MAWBP, EC:5.1.-.- |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC009738 |
| Gene Symbol | PBLD |
| Gene ID (NCBI) | 64081 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P30039 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PBLD antibody CL488-68317 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |