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PBLD Monoclonal antibody, PBS Only

PBLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11D4

Cat no : 68317-1-PBS

Print datasheet

Synonyms

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

验证数据展示

  • WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

    WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

  • WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

    WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

    IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

产品信息

68317-1-PBS targets PBLD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, pig
Immunogen PBLD fusion protein Ag23827 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phenazine biosynthesis-like protein domain containing
Synonyms FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD
Observed Molecular Weight30 kDa
GenBank Accession NumberBC009738
Gene Symbol PBLD
Gene ID (NCBI) 64081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30039
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)