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CoraLite® Plus 594 Anti-Human PD-1/CD279 (J110)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

J110

Cat no : CL594-65119

Print datasheet

Synonyms

CD279, PD1, PD-1, PD 1, J110

验证数据展示

  • FC experiment of human PBMCs using CL594-65119

    FC experiment of human PBMCs using CL594-65119

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone: J110) (red) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone:J110) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65119

    FC experiment of human PBMCs using CL594-65119

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with CL405 Anti-Human CD3 (CL405-65151, Clone: UCHT1) and 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone: J110) (right) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) (left). 1x10^6 untreated human PBMCs were surface stained with CL405 Anti-Human CD3 (CL405-65151, Clone:UCHT1) and 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone:J110) (center). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65119-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65119 targets PD-1/CD279 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, PD 1, J110
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 PD-1/CD279 antibody CL594-65119Download protocol
Standard Protocols
Click here to view our Standard Protocols