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验证数据展示

  • FC experiment of human PBMCs using FITC-65119

    FC experiment of human PBMCs using FITC-65119

    1x10^6 untreated (center) or PHA treated (3 day) human PBMCs were surface stained with CL750 Anti-Human CD3 (CL750-65151, Clone:UCHT1) and 5 ul FITC Plus Anti-Human PD-1/CD279 (FITC-65119, Clone:J110) (right) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21) (left). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65119

    FC experiment of human PBMCs using FITC-65119

    1x10^6 untreated (black dashed) or PHA treated (3 day) human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-1/CD279 (FITC-65119, Clone:J110) (red) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21) (blue). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

FITC Plus Anti-Human PD-1/CD279 (J110)

PD-1/CD279 Monoclonal Antibody for FC
Cat No. FITC-65119

产品说明书

CloneNo. J110

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

CD279, PD1, PD-1, PD 1, J110

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格: 

-/ -

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经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65119 targets PD-1/CD279 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, PD1, PD-1, PD 1, J110
计算分子量 288 aa, 32 kDa
GenBank蛋白编号BC074740
基因名称 PD-1
Gene ID (NCBI) 5133
RRIDAB_3673842
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for FITC Plus PD-1/CD279 antibody FITC-65119Download protocol
Standard Protocols
Click here to view our Standard Protocols