APC Anti-Human PD-L1 (B7-H1) (29E.2A3)
PD-L1 (B7-H1) Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b, kappa
Reactivity
Human and More (1)
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
29E.2A3
验证数据展示
经过测试的应用
| Positive FC detected in | human peripheral blood lymphocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 7 publications below |
产品信息
APC-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.
| Tested Applications | FC Application Description |
| Cited Applications | FC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | Full length human PD-L1 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD274 molecule |
| Synonyms | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1 (B7-H1), Programmed death ligand 1 |
| GenBank Accession Number | BC069381 |
| Gene Symbol | PD-L1 |
| Gene ID (NCBI) | 29126 |
| RRID | AB_2882979 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9NZQ7 |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC PD-L1 (B7-H1) antibody APC-65081 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy | ||
Oncogene Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells | ||
iScience Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis | ||
Stem Cell Res Ther Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment. | ||
Eur J Pharmacol PPARγ agonist pioglitazone enhances colorectal cancer immunotherapy by inducing PD-L1 autophagic degradation |
