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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PDHB Polyclonal antibody

PDHB Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14744

Print datasheet

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1

验证数据展示

  • IF Staining of HeLa using CL488-14744

    IF Staining of HeLa using CL488-14744

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PDHB antibody (CL488-14744) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-14744

    FC experiment of HeLa using CL488-14744

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PDHB Polyclonal antibody (CL488-14744)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDHB Ready-To-Use IHC Kit. PDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14744-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14744 targets PDHB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PDHB fusion protein Ag6442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) beta
Synonyms PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000439
Gene Symbol PDHB
Gene ID (NCBI) 5162
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11177
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
FC protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
Standard Protocols
Click here to view our Standard Protocols