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验证数据展示

  • IF Staining of HeLa using CL488-14744

    IF Staining of HeLa using CL488-14744

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PDHB antibody (CL488-14744) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-14744

    FC experiment of HeLa using CL488-14744

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PDHB Polyclonal antibody (CL488-14744)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PDHB Polyclonal antibody

PDHB Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14744

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDHB Ready-To-Use IHC Kit. PDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14744-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14744 targets PDHB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PDHB fusion protein Ag6442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) beta
别名 PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1
计算分子量 39 kDa
观测分子量 34 kDa
GenBank蛋白编号BC000439
基因名称 PDHB
Gene ID (NCBI) 5162
RRIDAB_3672603
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11177
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
FC protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
Standard Protocols
Click here to view our Standard Protocols