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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10221

Print datasheet

Synonyms

LMP1, LIM mineralization protein, ENIGMA

验证数据展示

  • IF Staining of SKOV-3 using CL488-10221

    IF Staining of SKOV-3 using CL488-10221

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite® Plus 488 PDLIM7 antibody (CL488-10221) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10221

    FC experiment of HeLa using CL488-10221

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pdlim7 Polyclonal Antibody (CL488-10221)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inSKOV-3 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10221-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10221 targets PDLIM7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen PDLIM7 fusion protein Ag0264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ and LIM domain 7 (enigma)
Synonyms LMP1, LIM mineralization protein, ENIGMA
Calculated Molecular Weight 55/60 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001093
Gene Symbol PDLIM7
Gene ID (NCBI) 9260
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR12
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDLIM7 antibody CL488-10221Download protocol
FC protocol for CL Plus 488 PDLIM7 antibody CL488-10221Download protocol
Standard Protocols
Click here to view our Standard Protocols