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验证数据展示

  • IF Staining of SKOV-3 using CL488-10221

    IF Staining of SKOV-3 using CL488-10221

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite® Plus 488 PDLIM7 antibody (CL488-10221) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10221

    FC experiment of HeLa using CL488-10221

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pdlim7 Polyclonal Antibody (CL488-10221)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10221

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

LMP1, LIM mineralization protein, ENIGMA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSKOV-3 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10221-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10221 targets PDLIM7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PDLIM7 fusion protein Ag0264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ and LIM domain 7 (enigma)
别名 LMP1, LIM mineralization protein, ENIGMA
计算分子量 55/60 kDa
观测分子量 55 kDa
GenBank蛋白编号BC001093
基因名称 PDLIM7
Gene ID (NCBI) 9260
RRIDAB_3672434
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR12
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDLIM7 antibody CL488-10221Download protocol
FC protocol for CL Plus 488 PDLIM7 antibody CL488-10221Download protocol
Standard Protocols
Click here to view our Standard Protocols